Choice of molecular barcode will affect species prevalence but not bacterial community composition

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• 作者：Karen Lebret^a ; ^b ; Joanna Schroeder^a ; Cecilia Balestreri^a ; Andrea Highfield^a ; Denise Cummings^c ; Tim Smyth^c ; Declan Schroeder^a ; ^{dsch@mba.ac.uk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Marine bacterial community ; 16S ; Pyrosequencing ; Illumina
• 刊名：Marine Genomics
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：29
• 期：Complete
• 页码：39-43
• 全文大小：482 K

文摘

The rapid advancement of next generation sequencing protocols in recent years has led to the diversification in the methods used to study microbial communities; however, how comparable the data generated from these different methods are, remains unclear. In this study we compared the taxonomic composition and seasonal dynamics of the bacterial community determined by two distinct 16s amplicon sequencing protocols: sequencing of the V6 region of the 16s rRNA gene using 454 pyrosequencing vs the V4 region of the 16s rRNA gene using the Illumina Hiseq 2500 platform. Significant differences between relative abundances at all taxonomic levels were observed; however, their seasonal dynamics between phyla were largely consistent between methods. This study highlights that care must be taken when comparing datasets generated from different methods.