文摘
The mutant ubiquitin UBB+1 is a substrate as well as an inhibitor of the ubiquitin-proteasome system (UPS) and accumulates in the neuropathological hallmarks of Alzheimer¡¯s disease (AD). A role for the UPS has been suggested in the generation of amyloid ¦Â (A¦Â) plaques in AD. To investigate the effect of UBB+1 expression on amyloid pathology in vivo, we crossed UBB+1 transgenic mice with a transgenic line expressing AD-associated mutant amyloid precursor protein (APPSwe) and mutant presenilin 1 (PS1dE9), resulting in APPPS1/UBB+1 triple transgenic mice. In these mice, we determined the A¦Â levels at 3, 6, 9 and 11 months of age. Surprisingly, we found a significant decrease in A¦Â deposition in amyloid plaques and levels of soluble A¦Â42 in APPPS1/UBB+1 transgenic mice compared to APPPS1 mice at 6 months of age, without alterations in UBB+1 protein levels or proteasomal chymotrypsin activity. These lowering effects of UBB+1 on A¦Â deposition were transient, as this relative decrease in plaque load was not significant in APPPS1/UBB+1 mice at 9 and 11 months of age. We also show that APPPS1/UBB+1 mice exhibit astrogliosis, indicating that they may not be improved functionally compared to APPPS1 mice despite the A¦Â reduction. The molecular mechanism underlying this decrease in A¦Â deposition in APPPS1/UBB+1 mice is more complex than previously assumed because UBB+1 is also ubiquitinated at K63 opening the possibility of additional effects of UBB+1 (e.g. kinase activation).