Chiral separation-based ligand exchange by open-tubular capillary electrochromatography

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• 作者：Cemil Aydo臒an ; Adil Denizli
• 关键词：Amino acid enantioseparation ; HPMA-Cl ; Ligand exchange ; Open-tubular CEC
• 刊名：Analytical Biochemistry
• 出版年：15 February, 2014
• 年：2014
• 卷：447
• 期：Complete
• 页码：55-57
• 全文大小：469 K

文摘

Chiral ligand-exchange enantioseparation of aliphatic and aromatic amino acids was successfully performed using a new open-tubular zwitterionic column with tentacle-type polymer stationary phase. The polymeric stationary phase was prepared using 3-chloro-2-hydroxypropyl methacrylate (HPMA-Cl), a new reactive monomer. The preparation procedure of the open-tubular column included silanization, in situ graft polymerization with HPMA-Cl, and l-histidine (l-His) modification. l-His was used as a chiral ligand-exchange selector and copper(II) as a central ion. Successful enantioseparation of six pairs of amino acid enantiomers was achieved with a buffer of 5 mM CuSO₄, 20 mM (NH₄)₂SO₄ at pH 3.0.