Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate
详细信息 查看全文
- 作者：Hans M.H. van Eijk ; Dennis P.L. Suylen ; Cornelis H.C. Dejong ; Yvette C. Luiking ; Nicolaas E.P. Deutz
- 关键词：Amino acid ; Stable isotope ; HPLC ; Mass spectrometry
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2007
- 出版时间：1 September 2007
- 年：2007
- 卷：856
- 期：1-2
- 页码：48-56
- 全文大小：269 K
文摘
An automated method is described to measure tracer-tracee-ratios (TTR) of plasma amino acids after their separation as 9-fluorenylmethylchloroformate derivatives. In a 45 min cycle, 5 μl plasma aliquots were derivatized, HPLC separated and subjected to electrospray ionization. By applying source collision induced dissociation, derivatives were dissociated at the peptide bond releasing the originating amino acids into the mass spectrometer. This approach enabled the determination of plasma amino acid TTRs with a standard deviation between 0.15 and 0.36 % , which is sufficient to study the fate of infused tracers and their conversion products in an in vivo experiment in humans.