Profiling of components and validated determination of iridoids in Gardenia Jasminoides Ellis fruit by a high-performance-thin-layer- chromatography/mass spectrometry approach
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- 作者：Silvia A. Coran ; Stefano Mulas ; Alessio Vasconi
- 关键词：HPTLC-densitometry ; HPTLC fingerprint ; TLC&ndash ; MS ; Gardenia Jasminoides (Zhi-zi) ; Iridoids ; Crocins ; Caffeoylquinic acid derivatives
- 刊名：Journal of Chromatography A
- 出版年：17 January, 2014
- 年：2014
- 卷：1325
- 期：Complete
- 页码：221-226
- 全文大小：730 K
文摘
A novel method was set up with the aim to obtain a simultaneous cross comparative evaluation of different Gardenia Jasminoides Ellis fruits by the HPTLC fingerprint approach. The main components among the iridoid, hydroxycinnamic derivative and crocin classes were identified by TLC-MS ancillary techniques. The iridoids geniposide, gardenoside and genepin-1-尾-d-gentiobioside were also quantitated by densitometric scanning at 240 nm. LiChrospher HPTLC Silica gel 60 RP-18 W F254, 20 cm 脳 10 cm plates with acetonitrile: formic acid 0.1% (40:60 v/v) as the mobile phase was used.
The method was validated giving rise to a dependable and high throughput procedure well suited to routine applications. Iridoids were quantified in the range of 240-1140 ng with RSD of repeatability and intermediate precision between 0.9-2.5% and accuracy with bias 1.6-2.6%. The method was tested on six commercial Gardenia Jasminoides fruit samples