Behavioral and genomic impacts of a wastewater effluent on the fathead minnow

详细信息    查看全文

• 作者：Natà ; lia Garcia-Reyero ; C ; ice M. Lavelle ; B. Lynn Escalon ; Dalma Martinović ; Kevin J. Kroll ; Peter W. Sorensen ; Nancy D. Denslow
• 关键词：Fathead minnow ; Effluent ; Genomics ; Microarrays ; Behavior
• 刊名：Aquatic Toxicology
• 出版年：2011
• 出版时间：17 January 2011
• 年：2011
• 卷：101
• 期：1
• 页码：38-48
• 全文大小：1320 K

文摘

Rivers containing effluents from water treatment plants are complex soups of compounds, ranging from pharmaceuticals to natural hormones. Male fathead minnows (Pimephales promelas) were exposed for 3 weeks to effluent waters from the Metropolitan Wastewater Treatment Plant in St. Paul, MN. Fish were tested for their competitive nest holding behavior. Changes in vitellogenin were measured and these were correlated to changes in gene expression using a 22,000 gene microarray developed specifically for fathead minnows. Significant changes in gene expression were observed in both liver and testis, which correlate to phenotypic changes of vitellogenin induction and reduced competitive behavior. We also compared by real-time PCR the expression changes in key genes related to steroid biosynthesis and metabolism in fish exposed to the effluent as well as in fish exposed to a model estrogen and a model androgen. While the gene expression signature from effluent-exposed fish shared some elements with estrogen and androgen signatures, overall it was different, underscoring the complexity of compounds present in sewage and their different modes of action.