Sexually dimorphic transcriptomic responses in the teleostean hypothalamus: A case study with the organochlorine pesticide dieldrin
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- 作者：Christopher J. Martyniuk ; cmartyn@unb.ca ; Nicholas J. Doperalski ; Kevin J. Kroll ; David S. Barber ; Nancy D. Denslow
- 关键词：A2M ; alpha-2-macroglobulin ; AMACR ; alpha-methylacyl-CoA racemase ; APOA1 ; apolipoprotein A-I ; AR ; androgen receptor ; BCL2L1 ; BCL2-like 1 ; CAT ; catalase ; CCNA2 ; cyclin A2 ; CCND2 ; cyclin D2 ; CCNE1 ; cyclin E1 ; CEL ; carboxyl ester lipase (bile salt-stimulated
- 刊名：Neurotoxicology
- 出版年：2013
- 出版时间：January, 2013
- 年：2013
- 卷：34
- 期：Complete
- 页码：105-117
- 全文大小：1306 K
文摘
Organochlorine pesticides (OCPs) such as dieldrin are a persistent class of aquatic pollutants that cause adverse neurological and reproductive effects in vertebrates. In this study, female and male largemouth bass (Micropterus salmoides) (LMB) were exposed to 3 mg dieldrin/kg feed in a 2 month feeding exposure (August-October) to (1) determine if the hypothalamic transcript responses to dieldrin were conserved between the sexes; (2) characterize cell signaling cascades underlying dieldrin neurotoxicity; and (3) determine whether or not co-feeding with 17¦Â-estradiol (E2), a hormone with neuroprotective roles, mitigates responses in males to dieldrin. Despite also being a weak estrogen, dieldrin treatments did not elicit changes in reproductive endpoints (e.g. gonadosomatic index, vitellogenin, or plasma E2). Sub-network (SNEA) and gene set enrichment analysis (GSEA) revealed that neuro-hormone networks, neurotransmitter and nuclear receptor signaling, and the activin signaling network were altered by dieldrin exposure. Most striking was that the majority of cell pathways identified by the gene set enrichment were significantly increased in females while the majority of cell pathways were significantly decreased in males fed dieldrin. These data suggest that (1) there are sexually dimorphic responses in the teleost hypothalamus; (2) neurotransmitter systems are a target of dieldrin at the transcriptomics level; and (3) males co-fed dieldrin and E2 had the fewest numbers of genes and cell pathways altered in the hypothalamus, suggesting that E2 may mitigate the effects of dieldrin in the central nervous system.