Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence

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• 作者：A. Watanabe^a ; ^{akari.watanabe@tokushima-u.ac.jp} ; N. Tamaki^b ; K. Yokota^c ; M. Matsuyama^a ; S. Kokeguchi^d
• 关键词：ATP bioluminescence ; Dental unit water line ; Bacteria ; Contamination ; Infection control
• 刊名：Journal of Hospital Infection
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：94
• 期：4
• 页码：393-396
• 全文大小：292 K
• 卷排序：94

文摘

Bacterial contamination of dental unit waterlines (DUWLs) was evaluated using ATP bioluminescence analysis and a conventional culture method. Water samples (N = 44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which gave counts ranging from 1.4×103 to 2.7×105 cfu/mL. The ATP bioluminescence results for DUWL samples ranged from 6 to 1189 relative light units and could be obtained within 1 min; these correlated well with the culture results (r = 0.727–0.855). We conclude that the results of the ATP bioluminescence assay accurately reflect the results of conventional culture-based testing. This method is potentially useful for rapid and simple monitoring of DUWL bacterial contamination.