Hypermethylation contributes to down-regulation of lysosomal 尾-hexosaminidase 伪 subunit in prostate cancer cells

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• 作者：Egidia Costanzi ; ^{egidia.costanzi@unipg.it" class="auth_mail} ; Lorena Urbanelli ; Ilaria Bellezza ; Alessandro Magini ; Carla Emiliani ; Alba Minelli
• 关键词：Ceramide ; Lysosomal glycohydrolases ; Hexosaminidase A ; Promoter methylation
• 刊名：Biochimie
• 出版年：June, 2014
• 年：2014
• 卷：101
• 期：Complete
• 页码：75-82
• 全文大小：1090 K

文摘

尾-Hexosaminidase, involved in degradation of glycoproteins and glycosphingolipids, is altered in several tumours leading to enhanced migration capacity. To date, the expression of the 尾-hexosaminidase isoenzymes in prostate cancer cells has not been elucidated. By using PC3, LNCaP, DUCaP, MDAPCa 2b, and hyperplasic prostate (BPH-1) cell lines, we analysed the 尾-hexosaminidase activity in each cell line and determined 尾-hexosaminidase 伪 subunit gene expression in PC3, LNCaP, and BPH-1. We then investigated the methylation status of the gene promoter and determined the cellular responses of PC3 and LNCaP after transfection with 尾-hexosaminidase 伪 subunit. We found that each prostate cancer cell line had a decrease in total hexosaminidase activity and that the lack of hexosaminidase A activity, observed in PC3 and LNCaP cells, was associated with mRNA disappearance. The HEXA promoter region in LNCaP and PC3 cell lines had methylated CpG islands, as confirmed by 5鈥?Aza-2鈥?deoxycitidine treatment, in PC3 cells, used as cell cancer model. We also tested, the involvement of hexosaminidase A in the migration capacity by migration assay using Hex 伪 subunit-transfected PC3. Finally, we found that, after Hex 伪 subunit transfection, both PC3 and LNCaP were less susceptible to exogenous ceramide treatment. Results indicate a likely contribution of the lysosomal enzyme to the acquisition of cancerous features.