Brain tissue oxygen evaluation by wireless near-infrared spectroscopy

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• 作者：Che-Chuan Wang ; MD^a ; ^b ; ^c ; ^d ; Jinn-Rung Kuo ; MD ; PhD^b ; ^c ; ^e ; Yu-Chih Chen ; BS^f ; Chung-Ching Chio ; MD^c ; Jhi-Joung Wang ; MD ; PhD^b ; Bor-Shyh Lin ; PhD^b ; ^f ; ^{borshyhlin@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Traumatic brain injury ; Partial pressure of oxygen in brain tissue ; Near-infrared spectroscopy ; Oxyhemoglobin ; Deoxyhemoglobin
• 刊名：Journal of Surgical Research
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：200
• 期：2
• 页码：669-675
• 全文大小：1191 K

文摘

Monitoring the partial pressure of oxygen in brain tissue (PbtO₂) is an important tool for traumatic brain injury (TBI) but is invasive and inconvenient for real time monitoring. Near-infrared spectroscopy (NIRS), which can monitor hemoglobin parameters in the brain tissue, has been used widely as a noninvasive tool for assessing cerebral ischemia and hypoxia. Therefore, it may have the potential as a noninvasive tool for estimating the change of PbtO₂. In this study, a novel wireless NIRS system was designed to monitor hemoglobin parameters of rat brains under different impact strengths and was used to estimate the change of PbtO₂ noninvasively in TBI.

Materials and methods

The proposed wireless NIRS system and a PbtO₂ monitoring system were used to monitor the oxygenation of rat brains under different impact strengths. Rats were randomly assigned to four different impact strength groups (sham, 1.6 atm, 2.0 atm, and 2.4 atm; n = 6 per group), and the relationships of concentration changes in oxyhemoglobin (HbO₂), deoxyhemoglobin (HbR), and total hemoglobin (HbT), and PbtO₂ during and after TBI with different impact strengths were investigated. Triphenyltetrazolium chloride (TTC) staining was also used to evaluate infarction volume.

Results

Concentration changes in HbO₂, HbR, and HbT dropped immediately after the impact, increased gradually, and then became stable. Changes in PbtO₂ had a similar tendency with the hemoglobin parameters. There was significant correlation between changes in PbtO₂ and HbO₂ (correlation = 0.76) but not with changes in HbR (correlation = 0.06). In triphenyltetrazolium chloride staining, the infarction volume was highly but negatively associated with oxygen-related parameters like PbtO₂ and HbO₂.

Conclusions

Changes in HbO₂ under TBI was highly and positively correlated with changes in PbtO₂. By using the relative changes in HbO₂ as a reference parameter, the proposed wireless NIRS system may be developed as a noninvasive tool for estimating the change of PbtO₂ in brain tissue after TBI.