Performance standard-based validation study for local lymph node assay: 5-bromo-2-deoxyuridine-flow cytometry method
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- 作者：Ilyoung Ahna ; Tae-Sung Kima ; Eun-Sun Jungb ; Jung-Sun Yib ; Won-Hee Jangc ; Kyoung-Mi Jungc ; Miyoung Parkc ; Mi-Sook Jungd ; Eun-Young Jeond ; Kyeong-uk Yeoe ; Ji-Hoon Joe ; Jung-Eun Parke ; Chang-Yul Kime ; Yeong-Chul Parke ; Won-Keun Seonga ; Ai-Young Leef ; Young Jin Chung ; Tae Cheon Jeongh ; Eui Bae Jeungi ; Kyung-Min Limj ; SeungJin Baej ; Soojung Sohna ; Yong Heoe ; yheo@cu.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Performance standards ; Validation study ; Local lymph node assay ; LLNA ; BrdU-FCM ; Skin sensitization ; Flow cytometry
- 刊名：Regulatory Toxicology and Pharmacology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：183-194
- 全文大小：1087 K
文摘
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The reliability and accuracy of the LLNA:BrdU-FCM were evaluated according to the OECD TG 429 PS and Guidance Document 34.
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5">The LLNA:BrdU-FCM protocol has been optimized through inclusion of a delicate pre-screen test and solubility test process.
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20">The predictive capacity was assessed and the accuracy of the test method was 88.9%.