Filter strip as a method of choice for apoplastic fluid extraction from maize roots
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- 作者：Jelena J. Dragi&scaron ; i膰 Maksimovi膰a ; draxy@imsi.rs" class="auth_mail ; draxy@imsi.bg.ac.rs" class="auth_mail ; Branka D. 沤ivanovi膰a ; vunduk@imsi.bg.ac.rs" class="auth_mail ; Vuk M. Maksimovi膰a ; maxivuk@imsi.bg.ac.rs" class="auth_mail ; Milo&scaron ; D. Mojovi膰b ; milos@ffh.bg.ac.rs" class="auth_mail ; Miroslav T. Nikolica ; mnikolic@imsi.bg.ac.rs" class="auth_mail ; 沤eljko B. Vu膷ini膰a ; vucinic@imsi.bg.ac.rs" class="auth_mail
- 关键词：AF ; apoplastic fluid ; AWF ; apoplastic washing fluid ; DEPMPO ; 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide ; DEPMPO/OH ; DEPMPO adduct with hydroxyl radical ; EPR ; spin trapping electron paramagnetic resonance ; G-6-P ; glucose-6-phosphate ; IEF ; isoelectric focusing ; MDH ; malate dehydrogenase ; OH ; hydroxyl radical ; PPB ; potassium phosphate buffer ; POD ; peroxidase (EC 1.11.1.7) ; SOD ; superoxide dismutase (EC 1.15.1.1).
- 刊名：Plant Science
- 出版年：June 2014
- 年：2014
- 卷：223
- 期：Complete
- 页码：49-58
- 全文大小：1274 K
文摘
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Apoplastic washing fluid and fluid extracted by filter paper strips were compared.
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Cytoplasmic markers and antioxidative components of the extracts were analyzed.
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Filter strips method enables small amounts of concentrated and uncontaminated fluid.
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This is an advanced method that avoids cytoplasmic contamination and dilution.
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Filter strips facilitate precise analysis of metabolites in root apoplastic fluid.