Constructing liposomal nanovesicles of ginseng extract against hydrogen peroxide-induced oxidative damage to L929 cells

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• 作者：Wen-Che Tsai^a ; Wei-Chu Li^b ; Hsin-Yi Yin^a ; Ming-Chiang Yu^a ; Hsiao-Wei Wen^a ; ^{hwwen@nchu.edu.tw}
• 关键词：Ginseng extract ; Antioxidant activity ; Liposomal nanovesicle ; Mitochondrial membrane potential ; Apoptosis
• 刊名：Food Chemistry
• 出版年：2012
• 出版时间：15 May 2012
• 年：2012
• 卷：132
• 期：2
• 页码：744-751
• 全文大小：327 K

文摘

In this study, a liposomal nanovesicle was applied as a nanocarrier of ginseng extract (GE) to enhance the intracellular antioxidant activity of GE. The optimal condition of preparing GE liposomal nanovesicle (GELN) was prepared by tagging with 3.0 mol % of polyethenyl glycol (PEG3.0) followed by homogenisation at 15,000 psi for 5 min to reduce its diameter to ?50 nm. PEG3.0-GELN encapsulated the most ginsenosides (234.1 ¡À 13.9 mg/g) with a higher reducing power than GELNs tagged with lower amounts of PEG (0-1.5 mol % ). After treatment with PEG3.0-GELN (20 ¦Ìg ginsenoside/ml), the % increase in mitochondrial membrane potential (¦¤¦·) of H₂O₂-damaged cells rose 20.9 % and the % decrease in apoptosis increased 12.4 % , compared with those treated with GE. Therefore, this investigation proved that utilising liposomal nanovesicles to encapsulate GE can effectively suppress the depolarisation of ¦¤¦· and thus enhance the survival rate of H₂O₂-damaged cells.