Cellular ROS imaging with hydro-Cy3 dye is strongly influenced by mitochondrial membrane potential

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• 作者：Alexander V. Zhdanov^a ; ^{a.zhdanov@ucc.ie} ; Gabriella Aviello^b ; ^c ; Ulla G. Knaus^b ; Dmitri B. Papkovsky^a
• 关键词：ROS probes ; Hydrocyanines ; Hydro-Cy3 ; Mitochondrial membrane potential ; Live cell imaging
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1861
• 期：2
• 页码：198-204
• 全文大小：1699 K
• 卷排序：1861

文摘

A critical drawback of common ROS probes hydrocyanines is described. When oxidised by ROS, intracellular hydro-Cy3 accumulates in polarised mitochondria. Cy3 signal dissipates and loses brightness upon mitochondrial depolarisation Cy3 may misinform on the quantity of ROS and their main production sites in the cell. Control of mitochondrial polarisation state is mandatory when using hydrocyanines.