Early and sharp nitric oxide production and anoxic depolarization in the rat hippocampus during transient forebrain ischemia

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• 作者：Min Hai Jiang ; Junichi Hada
• 关键词：Anoxic depolarization ; Cerebral ischemia ; Nitric oxide ; Nitric oxide synthase inhibitor ; Nitric oxide scavenger
• 刊名：European Journal of Pharmacology
• 出版年：2007
• 出版时间：12 July 2007
• 年：2007
• 卷：567
• 期：1-2
• 页码：83-88
• 全文大小：217 K

文摘

This study was designed to characterize nitric oxide (NO) production and anoxic depolarization in the rat hippocampus during transient forebrain ischemia using two NO synthase (NOS) inhibitors, l-N⁵-(1-iminoethyl)ornithine (l-NIO), a relatively selective endothelial NOS (eNOS) inhibitor, and 7-nitroindazole, a relatively selective neuronal NOS (nNOS) inhibitor, and an NO scavenger, [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide] (carboxy-PTIO). We measured the mean arterial blood pressure, hippocampal blood flow, NO concentration and direct current potential before, during and after transient forebrain ischemia, which was induced by 4-vessel occlusion for 10 min. Saline, l-NIO (20 mg/kg), 7-nitroindazole (25 mg/kg), l-NIO (20 mg/kg) + 7-nitroindazole (25 mg/kg) or carboxy-PTIO (1 mg/kg) was administered intraperitoneally 20 min before the onset of ischemia. We observed early and sharp NO production in the hippocampus during ischemia in the saline group. This NO increase during ischemia was significantly reduced by l-NIO (20 mg/kg) + 7-nitroindazole (25 mg/kg) or carboxy-PTIO (1 mg/kg), but not l-NIO (20 mg/kg) or 7-nitroindazole (25 mg/kg). On the other hand, NO production after ischemia was significantly reduced by 7-nitroindazole (25 mg/kg), l-NIO (20 mg/kg) + 7-nitroindazole (25 mg/kg) or carboxy-PTIO (1 mg/kg), but not l-NIO (20 mg/kg). The peak latency of NO production during ischemia always preceded the onset latency of anoxic depolarization in both the saline group and the carboxy-PTIO group. In the carboxy-PTIO group, the onset latency of anoxic depolarization was significantly longer than that in the saline group. Moreover, carboxy-PTIO significantly reduced the anoxic depolarization amplitude, compared with that of the saline group. These results suggest that both NOS-dependent and-independent NO formation contributes to early and sharp NO production during ischemia, and that this NO increase is, at least in part, related to the triggering of anoxic depolarization.