Ectopic expression of ZmSIMK1 leads to improved drought tolerance and activation of systematic acquired resistance in transgenic tobacco
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- 作者：Li Wang ; Yang Liu ; Guohua Cai ; Shanshan Jiang ; Jiaowen Pan ; Dequan Li
- 关键词：MAPK ; mitogen-activated protein kinase ; qRT-PCR ; quantitative real-time PCR ; RT-PCR ; reverse transcription PCR ; Pst DC3000 ; Pseudomonas syringae pv. tomato DC3000 ; ROS ; reactive oxygen species ; PR ; pathogen related genes ; SAR ; systemic acquired resistance ; ABA ; abscisic acid ; SA ; salicylic acid ; MeJA ; methyl jasmonate ; GFP ; green fluorescent protein ; DAPI ; 4&prime ; 6-dianidino-2 phenylindole ; CD ; C-terminal domain ; KD ; kinase domain ; REL ; relative electrolyte leakage ; MDA ; malondialdehyde ; NBT ; n
- 刊名：Journal of Biotechnology
- 出版年：20 February, 2014
- 年：2014
- 卷：172
- 期：Complete
- 页码：18-29
- 全文大小：3717 K
文摘
The mitogen-activated protein kinase (MAPK) cascades play pivotal roles in diverse signaling pathways related to plant biotic and abiotic stress responses. In this study, a group B MAPK gene in Zea mays, ZmSIMK1, was functionally analyzed. Quantitative real-time PCR (qRT-PCR) analysis indicated that ZmSIMK1 transcript could be induced by drought, salt, Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and certain exogenous signaling molecules. Analysis of the ZmSIMK1 promoter revealed a group of putative cis-acting elements related to drought and defense responses. 尾-Glucuronidase (GUS) staining produced similar results as qRT-PCR. ZmSIMK1 was mainly localized in the nucleus, and further study indicated that the C-terminal domain (CD) was essential for targeting to the nucleus. Transgenic tobacco accumulated less reactive oxygen species (ROS), had higher levels of antioxidant enzyme activity and osmoregulatory substances and exhibited an increased germination rate compared with wild-type (WT) tobacco under drought stress. ROS-related and drought stress-responsive genes in transgenic tobacco were significantly upregulated compared with the same genes in WT lines under drought stress. Moreover, overexpression of ZmSIMK1 promoted the hypersensitive response (HR) and pathogen-related gene (PR) transcription in addition to triggering systemic acquired resistance (SAR) in tobacco.