Effect of Emdogain enamel matrix derivative and BMP-2 on the gene expression and mineralized nodule formation of alveolar bone proper-derived stem/progenitor cells

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• 作者：Karim M. Fawzy El-Sayed^a ; ^b ; ^{karim.fawzy@gmail.com" class="auth_mail} ; Christof Dö ; rfer^a ; Hendrick Ungefroren^c ; Neemat Kassem^d ; Jö ; rg Wiltfang^e ; Sebastian Paris^f
• 关键词：Stem cells ; Tissue engineering ; Alveolar bone proper ; EMD ; BMP-2
• 刊名：Journal of Cranio-Maxillofacial Surgery
• 出版年：July 2014
• 年：2014
• 卷：42
• 期：5
• 页码：568-576
• 全文大小：2966 K

文摘

The objective of this study was to evaluate the effect of Emdogain (Enamel Matrix Derivative, EMD) and Bone Morphogenetic Protein-2 (BMP-2), either solely or in combination, on the gene expression and mineralized nodule formation of alveolar bone proper-derived stem/progenitor cells.

Stem/progenitor cells were isolated from human alveolar bone proper, magnetically sorted using STRO-1 antibodies, characterized flowcytometrically for their surface markers' expression, and examined for colony formation and multilineage differentiation potential. Subsequently, cells were treated over three weeks with 100 渭g/ml Emdogain (EMD-Group), or 100 ng/ml BMP-2 (BMP-Group), or a combination of 100 ng/ml BMP-2 and 100 渭g/ml Emdogain (BMP/EMD-Group). Unstimulated stem/progenitor cells (MACS⁺-Group) and osteoblasts (OB-Group) served as controls. Osteogenic gene expression was analyzed using RTq-PCR after 1, 2 and 3 weeks (N = 3/group). Mineralized nodule formation was evaluated by Alizarin-Red staining.

BMP and EMD up-regulated the osteogenic gene expression. The BMP Group showed significantly higher expression of Collagen-I, III, and V, Alkaline phosphatase and Osteonectin compared to MACS⁺- and OB-Group (p < 0.05; Two-way ANOVA/Bonferroni) with no mineralized nodule formation.

Under in-vitro conditions, Emdogain and BMP-2 up-regulate the osteogenic gene expression of stem/progenitor cells. The combination of BMP-2 and Emdogain showed no additive effect and would not be recommended for a combined clinical stimulation.