Biotransformation of prednisone and dexamethasone by cytochrome P450 based systems - Identification of new potential drug candidates

详细信息    查看全文

• 作者：Natalia Putkaradze^a ; ¹ ; Flora Marta Kiss^a ; ¹ ; Daniela Schmitz^a ; Josef Zapp^b ; Michael C. Hutter^c ; Rita Bernhardt^a ; ^{ritabern@mx.uni-saarland.de}
• 关键词：Prednisone ; Dexamethasone ; Hydroxylation ; CYP106A2 ; Biocatalysis ; Bacillus megaterium
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：101-110
• 全文大小：1668 K
• 卷排序：242

文摘

The ability of CYP106A2 to hydroxylate prednisone and dexamethasone at position 15β is described. The in vitro conversion of prednisone and dexamethasone with CYP106A2 is thoroughly characterized. CYP106A2-based whole-cell systems in Bacillus megaterium are applied for the biotransformation of prednisone and dexamethasone. The identified prednisone and dexamethasone derivatives are promising candidates for the development of novel steroidal drugs.