Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation

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• 作者：M. Dioní ; sio^a ; ^{maritadionisio@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; L. Braz^b ; ^c ; ^d ; ^{lvbraz@ualg.pt" class="auth_mail" title="E-mail the corresponding author} ; M. Corvo^e ; ^{marta.corvo@fct.unl.pt" class="auth_mail" title="E-mail the corresponding author} ; J.P. Lourenç ; o^b ; ^f ; ^g ; ^{jlouren@ualg.pt" class="auth_mail" title="E-mail the corresponding author} ; A. Grenha^a ; ^f ; ^{amgrenha@ualg.pt" class="auth_mail" title="E-mail the corresponding author} ; A.M. Rosa da Costa^b ; ^f ; ^{amcosta@ualg.pt" class="auth_mail" title="E-mail the corresponding author}
• 关键词：(3&ndash ; 6) ; Sulfated pullulan ; Tetraalkylammonium pullulan ; Polyelectrolyte complexation
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：86
• 期：Complete
• 页码：129-138
• 全文大小：1908 K

文摘

Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO₃^.DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on ¹H- and ¹³C NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation.