Biotransformation of methyl parathion by marine-derived fungi isolated from ascidian Didemnum ligulum

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• 作者：Gisele N. Rodrigues^a ; Natá ; lia Alvarenga^b ; Bruna Vacondio^a ; Suzan P. de Vasconcellos^c ; Michel R.Z. Passarini^c ; Mirna H.R. Seleghim^a ; André ; L.M. Porto^b ; ^{almporto@iqsc.usp.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Organophosphate pesticide ; Biodegradation ; Marine fungi ; p-Nitrophenol
• 刊名：Biocatalysis and Agricultural Biotechnology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：24-30
• 全文大小：1393 K

文摘

This paper describes the biotransformation of methyl parathion by marine-derived fungi isolated from ascidian Didemnum ligulum. Seventeen strains were screened by their growth in solid culture medium containing different concentrations of methyl parathion (120 mg L⁻¹, 240 mg L⁻¹ and 360 mg L⁻¹). Three strains with potential to degrade methyl parathion were identified as Penicillium citrinum (DL4M3), P. citrinum (DL9M3) and Fusarium proliferatum (DL11A). When selected for experiments in liquid medium to quantify the biodegradation of methyl parathion, they were able to completely degrade it in 20 d. However, when comparing the hydrolysis of methyl parathion catalyzed by fungi with the chemical hydrolysis (abiotic control), there was no significant differences in the disappearance of the pesticide in both reactions. On the other hand, F. proliferatum DL11A strain was able to reduce the concentration of p-nitrophenol, the main metabolite of methyl parathion hydrolysis, promoting 90% of biodegradation/bioconjugation of p-nitrophenol in 30 d.