High-level production of aquaporin Z in Escherichia coli using maltose-binding protein/polyhistidine dual-affinity tag fusion system
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- 作者：Baojian Hanga ; Jianfeng Pana ; Desheng Nic ; Qiang Zhenga ; b ; Xu Zhanga ; b ; Jin Caia ; b ; Lei Huanga ; b ; Peilian Weid ; weipeilian@126.com" class="auth_mail" title="E-mail the corresponding author ; Zhinan Xua ; b ; znxu@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Aquaporin Z ; MBP/polyhistidine dual-affinity tag system ; Membrane-associated expression ; Membrane protein
- 刊名：Process Biochemistry
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：51
- 期：5
- 页码：599-606
- 全文大小：1630 K
文摘
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Aquaporin Z (AqpZ) was expressed as the fusion protein maltose-binding protein (MBP)–AqpZ–polyhistidine (HIS).
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The MBP tag of MBP–AqpZ–HIS was removed by factor Xa.
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AqpZ–HIS was purified using affinity chromatography.
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The yield of functional AqpZ–HIS was 15–20 mg/l.