Efficient induction of cross-presentating human B cell by transduction with human adenovirus type 7 vector
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- 作者：Ying Penga ; 1 ; 2 ; Meimei Laia ; b ; c ; Yunyan Loua ; b ; c ; Yanqing Liua ; b ; c ; Huiyan Wanga ; b ; c ; Xiaoqun Zhenga ; b ; c ; 1 ; jszhengxq@63.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：APC ; antigen-presenting cell ; ARD ; acute respiratory disease ; APM ; antigen-processing machinery ; CEA ; carcinoembryonic antigen ; CPE ; cytopathic effect ; Ct ; threshold cycle ; CTL ; cytotoxic T lymphocytes ; DC ; dendritic cell ; DSG-2 ; desmoglein 2 ; ELISA ; enzyme-linked immunosorbent assay ; GFP ; green fluorescent protein ; HRP ; horseradish peroxidase ; HAdV7 ; human adenovirus type 7 ; hCAR ; human coxsackievirus-adenovirus receptor ; ODN ; oligonucleotide ; PBMC ; peripheral blood mononuclear cell ; p.i ; post
- 刊名：Immunology Letters
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：169
- 期：Complete
- 页码：41-51
- 全文大小：2487 K
文摘
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We demonstrated naive human B cells can be efficiently transduced by recombinant HAdV7.
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We observed HAdV7 transduction increased the expression of molecules involved in human B cell cross-presentation.
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We found that CEA-specific CD8+ T cells are induced by HAd7CEA modified autologous B cells.
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20">We proved that HAdV7-initiated TLR9 signaling is involved in and necessary for effective B cell cross-presentation.