Evaluation of matrix-assisted laser desorption/ionization time-of-fight mass spectrometry for identification of 345 clinical isolates of Aspergillus species from 11 Korean hospitals: comparison with molecular identification

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• 作者：Ju Heon Park^a ; Jong Hee Shin^a ; ^{shinjh@chonnam.ac.kr} ; Min Ji Choi^a ; Jin Un Choi^a ; Yeon-Joon Park^b ; Sook Jin Jang^c ; Eun Jeong Won^a ; Soo Hyun Kim^a ; Seung Jung Kee^a ; Myung Geun Shin^a ; Soon Pal Suh^a
• 关键词：Aspergillus ; MALDI-TOF MS ; MALDI Biotyper ; Identification
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：87
• 期：1
• 页码：28-31
• 全文大小：224 K
• 卷排序：87

文摘

We evaluated the ability of the Filamentous Fungi Library 1.0 of the MALDI-TOF MS Biotyper system to identify 345 clinical Aspergillus isolates, by comparing the results with those of sequence-based identification. Compared with results of the internal transcribed spacer region sequencing, the frequencies of correct identification at the species-complex level were 94.5% and 98.8% with cutoff values of 2.0 and 1.7, respectively. Compared with results of β-tubulin gene sequencing, the frequencies of correct identification at the species level were 96.0% (cutoff 2.0) and 100% (cutoff 1.7) for 303 Aspergillus isolates of five common, non-cryptic species, but only 4.8% (cutoff 1.7) and 0% (cutoff 2.0) for 42 Aspergillus isolates of six cryptic species. The MALDI Biotyper using the Filamentous Fungi Library version 1.0 enables reliable identification of the majority of common clinical Aspergillus isolates, although the database should be expanded to facilitate identification of cryptic species.)