Ultrasensitive caspase-3 activity detection using an electrochemical biosensor engineered by gold nanoparticle functionalized MCM-41: Its application during stem cell differentiation
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- 作者：Balal KhalilzadehaAuthor Vitae ; Hojjatollah Nozad Charoudehb ; cAuthor Vitae ; Nasrin ShadjoudAuthor Vitae ; Rahim Mohammad-RezaeieAuthor Vitae ; Yadollah Omidia ; fAuthor Vitae ; Kobra Velaeib ; cAuthor Vitae ; Zeinab Aliyarib ; cAuthor Vitae ; Mohammad-Reza Rashidia ; f ; rashidi@tbzmed.ac.ir" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：MCM-41 ; Electrochemical biosensor ; Gold nanoparticle ; Caspase-3 ; Stem cell differentiation ; Apoptosis
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：231
- 期：Complete
- 页码：561-575
- 全文大小：5368 K
文摘
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An AuNPs-S-MCM-41 based biosensor has been developed for ultrasensitive measurement of caspase-3 activity.
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The electrocatalytic activity of the designed biosensor was investigated with various electrochemical techniques.
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The developed biosensor was able to detect caspase-3 activity in its active form with LLOQ of 5 fM.
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The biosensor was evaluated by detection of caspase-3 activity for 21 day during stem cell differentiation process.