Optical detection of neuron connectivity by random access two-photon microscopy
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- 作者：Nasrin Shafeghata ; 1 ; Morteza Heidarinejada ; Noboru Muratab ; Hideki Nakamuraa ; 2 ; Takafumi Inouea ; inoue.t@waseda.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Voltage sensitive dye ; Random access two-photon microscopy ; Population spike recording ; Neural connectivity
- 刊名：Journal of Neuroscience Methods
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：263
- 期：Complete
- 页码：48-56
- 全文大小：1862 K
文摘
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Voltage sensitive dye and a random-access two-photon microscope were combined to detect action potentials in multiple cultured neurons.
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Long-duration recording up to 100 min yielded enough number of spike events for analysis of synaptic connections.
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Cross-correlation analysis of neuron pairs clearly distinguished synaptically connected neuron pairs and the direction of connection.