Zwitterionic hydrophilic interaction solid-phase extraction and multi-dimensional mass spectrometry for shotgun lipidomic study of Hypophthalmichthys nobilis
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- 作者：Renyao Jina ; 1 ; Linqiu Lib ; 1 ; Junli Fenga ; Zhiyuan Daia ; Yao-Wen Huangc ; Qing Shena ; leonqshen@163.com" class="auth_mail" title="E-mail the corresponding author ; sq@zjgsu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：PC ; Phosphatidylcholine ; PE ; Phosphatidylethanolamine ; PI ; Phosphatidylinositol ; PS ; Phosphatidylserine ; MS ; Mass spectrometry ; MDMS ; Multi-dimensional mass spectrometry ; FAC ; Fatty acyl chain ; ZIC ; Zwitterionic ; HILIC ; Hydrophilic interaction liquid chromatography ; PIS ; Product ion scan ; NLS ; Neutral loss scan ; SPE ; Solid-phase extraction ; LOD ; Limit of detection ; LOQ ; Limit of quantification ; SM ; sphingomyelin ; S/N ; signal-to-noise ; MCA ; Multiple-channel acquisition
- 刊名：Food Chemistry
- 出版年：2017
- 出版时间：1 February 2017
- 年：2017
- 卷：216
- 期：Complete
- 页码：347-354
- 全文大小：1575 K
文摘
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Phospholipids were purified using a ZIC-HILIC SPE cartridge.
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Molecular species of each phospholipid are studied by shotgun lipidomics.
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Phospholipids with polyunsaturated acyl chains were detected in H. nobilis.
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The method was validated and shown to be reliable and efficient.