Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes

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• 作者：Yi-Tong Li^a ; Yi-Chao Huang^b ; ^{hycjimmy@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Yang Xu^c ; Man Pan^b ; Yi-Ming Li^b ; ^{lym2007@mail.ustc.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ub-ACC ; Deubiquitinating enzyme ; Ub-AMC ; Peptide hydrazides ; Protease assay
• 刊名：Tetrahedron
• 出版年：2016
• 出版时间：7 July 2016
• 年：2016
• 卷：72
• 期：27-28
• 页码：4085-4090
• 全文大小：794 K

文摘

A new fluorogenic substrate Ub-ACC (ubiquitin C-terminal 7-amino-4-carbamoylmethyl-coumarin) was developed for DUB (deubiquitinating enzyme) activity assays. This substrate can be synthesized with higher efficiency than the classical DUB substrate, Ub-AMC (ubiquitin C-terminal 7-amino-4-methylcoumarin). DUB assays using UCH-L3, OTUD2 and USP30 demonstrated that Ub-ACC shows nearly 2-fold higher sensitivity than Ub-AMC.