Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles
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- 作者：Christopher Ladd Effioa ; Tobias Hahna ; Julia Seilera ; Stefan A. Oelmeiera ; b ; Iris Asenc ; Christine Silbererc ; Louis Villaind ; Jü ; rgen Hubbucha ; juergen.hubbuch@kit.edu" class="auth_mail" title="E-mail the corresponding author ;
- 关键词：Quality by design ; Membrane chromatography ; Predictive modeling ; Downstream processing ; Virus-like particle vaccine ; High-throughput process development
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：1429
- 期：Complete
- 页码：142-154
- 全文大小：1554 K
文摘
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Purification of human B19 parvovirus-like particles by anion-exchange membrane chromatography.
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High-throughput screening-based selection of VLP binding conditions.
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Simulation of radial IEX membrane chromatography for a complex biological feedstock.
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In silico optimization of a VLP bind-and-elute process applying stoichiometric displacement model.