High-throughput process development of an alternative platform for the production of virus-like particles in Escherichia coli
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- 作者：Christopher Ladd Effioa ; 1 ; christopher.ladd@kit.edu" class="auth_mail" title="E-mail the corresponding author ; Pascal Baumanna ; 1 ; pascal.baumann@kit.edu" class="auth_mail" title="E-mail the corresponding author ; Claudia Weigela ; Philipp Vormittaga ; Anton Middelbergb ; Jü ; rgen Hubbucha ; juergen.hubbuch@kit.edu" class="auth_mail" title="E-mail the corresponding author ;
- 关键词：Virus-like particles ; Escherichia coli ; Micro-scale cultivation ; BioLector ; High-throughput screening
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 February 2016
- 年：2016
- 卷：219
- 期：Complete
- 页码：7-19
- 全文大小：3901 K
文摘
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Establishing a HTS workflow for cell cultivation, lysis and analytics.
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Optimizing the production of recombinant virus-like particles for chimeric vaccines.
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Yielding 1.63 mg/mL VP1 with 43% TSP by codon optimization and design of experiments.
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Developing a simple two-step downstream process for murine polyomavirus protein VP1.
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Assembling VP1 into homogeneous VLPs with a size of 40–50 nm and a protein purity of 92%.