microRNA expression profiling and functional annotation analysis of their targets modulated by oxidative stress during embryonic heart development in diabetic mice
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- 作者：Daoyin Donga ; Yuji Zhangb ; E. Albert Reecea ; d ; Lei Wangc ; Christopher R. Harmana ; Peixin Yanga ; d ; pyang@fpi.umaryland.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pregestational diabetes mellitus ; Congenital heart defects ; Diabetic embryopathy ; miRNA profiling ; Heart development ; Oxidative stress
- 刊名：Reproductive Toxicology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：65
- 期：Complete
- 页码：365-374
- 全文大小：2864 K
文摘
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miRNA microarray identified 149 of significantly altered miRNAs in the diabetic embryonic heart.
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2111 of high-confidence miRNA targets were generated by a two-step bioinformatics approach and gene ontology indicated that the majority of the targets were associated with transcription.
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Cardiac development-related pathways including STAT3 and IGF-1 pathways and transcription factors, for example, Cited2, Zeb2, Mef2c, Smad4 and Ets1, were altered by PGDM.
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PGDM-induced oxidative stress leads to the alterations of miRNAs and their targets in developing embryonic heart.