Accessing regio-and typo-selectivity of Yarrowia lipolytica lipase in its free form and immobilized onto magnetic nanoparticles
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- 作者：Emí ; lia Akila ; Tamires Carvalhob ; Bruno Bá ; reac ; Priscilla Finotellid ; Jé ; rô ; me Lecomtec ; Alexandre G. Torresa ; Priscilla Amaralb ; pamaral@eq.ufrj.br" class="auth_mail" title="E-mail the corresponding author ; Pierre Villeneuvec
- 关键词：DAG ; diacylglycerol ; FA ; fatty acid ; FAEEs ; fatty acid ethyl esters ; FAMEs ; fatty acid methyl esters ; FID ; flame ionization detector ; FFA ; free fatty acid ; GC ; gas chromatography ; GRAS ; generally recognized as safe ; MAG ; monoacylglycerol ; MUFAs ; monounsaturated fatty acids ; p-NPL ; p-nitrophenyl laurate ; PUFAs ; polyunsaturated fatty acids ; PVA ; polyvinyl alcohol ; SmF ; submerged fermentation ; TAG ; triacylglycerol ; TLC ; thin layer chromatography ; YPD ; yeast extract ; peptone ; dextrose
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：109
- 期：Complete
- 页码：101-111
- 全文大小：2031 K
文摘
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Extracellular lipase from Y. lipolytica IMUFRJ 50682 is strictly 1,3-regioselective.
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Immobilization onto magnetic nanoparticules reduced the 1,3-regioselectivity.
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Immobilization on nano-sized magnetic particles increased lipase stability to pH and T.
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Free or immobilized lipases were able to hydrolyze saturated esters.
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Free lipase from Y. lipolytica is typo-selective towards long-chain unsaturated FA.