Influence of Pichia pastoris cellular material on polymerase chain reaction performance as a synthetic biology standard for genome monitoring
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- 作者：Alexander Templar ; Stefan Woodhouse ; Eli Keshavarz-Moore ; Darren N. Nesbeth ; d.nesbeth@ucl.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：PCR ; polymerase chain reaction ; BMGY ; buffered glycerol complex medium ; e-pPCR ; end-point PCR ; HCD ; high cell density ; qPCR ; quantitative PCR ; YPD ; yeast extract peptone dextrose ; SF ; shake flask ; wcw ; wet cell weight ; WCB ; working cell bank ; LRE ; linear regression of efficiency ; OCF ; optical calibration factor
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：127
- 期：Complete
- 页码：111-122
- 全文大小：1386 K
文摘
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Quantification of the effect of P. pastoris cellular material on sensitivity of end-point PCR
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Evaluation of the effect of P. pastoris cellular material on accuracy of qPCR
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Demonstration of LRE qPCR as equivalent to ‘standard curve’ qPCR.
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Proposal of the CAL1 reaction as a synthetic biology standard for qPCR analysis of P. pastoris cellular material