Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein
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- 作者：Gui-Hong Cha1 ; Yuan Liu1 ; Ting Peng ; Ming-Zhu Huang ; Chen-Ying Xie ; Yu-Chao Xiao ; Wei-Na Wang ; wangwn@scnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; weina63@aliyun.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：P. pastoris ; Pichia pastoris ; L. vannamei (Lv) ; Litopenaeus vannamei ; V. alginolyticus ; Vibrio alginolyticus ; B. subtilis ; Bacillus subtilis ; S. aureus ; Staphylococcus aureus ; A. veronii ; Aeromonas veronii ; BSA ; bovine serum albumin ; YPD ; yeast extract peptone dextrose medium ; BMGY ; buffered minimal glycerol-complex medium ; BMMY ; buffered minimal methanol-complex medium ; YPDS ; yeast extract peptone dextrose sorbitol medium ; TBST ; Tris-buffered saline with 0.05% Tween 20 ; PVDF ; polyvinylidene dif
- 刊名：Molecular Immunology
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：67
- 期：2
- 页码：325-340
- 全文大小：3916 K
文摘
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Litopenaeus vannamei galectin was the first expressed in Pichia pastoris.
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Transcript of Lvgalectin was markedly increased after challenge with Vibrio alginolyticus.
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The recombinant protein rLvgalectin has bacteria binding and antimicrobial activities, and enhance V. alginolyticus clearance in vivo.
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Suppression of Lvgalectin significantly increased the transcripts of proPO, Crustin, p53, and penaeidin3 after challenged with V. alginolyticus.
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The Lvgalectin silencing increased a cumulative mortality in Vibrio alginolyticus injected shrimps in comparison to the control groups.