Optimization of selenylation conditions for lycium barbarum polysaccharide based on antioxidant activity

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• 作者：Shulei Qiu ; Jin Chen ; Xi Chen ; Qiang Fan ; Cunshuai Zhang ; Deyun Wang ; Xiuping Li ; Xingying Chen ; Xiaolan Chen ; Cui Liu ; Zhenzhen Gao ; Hongquan Li ; Yuanliang Hu
• 关键词：Selenizing lycium barbarum polysaccharide ; Free radical-scavenging rate ; GSH-Px activity ; SOD activity ; MDA content
• 刊名：Carbohydrate Polymers
• 出版年：15 March, 2014
• 年：2014
• 卷：103
• 期：Complete
• 页码：148-153
• 全文大小：583 K

文摘

Lycium barbarum polysaccharide (LBP) was modified by HNO₃-Na₂SeO₃ method according to L₉(3⁴) orthogonal design to obtain nine selenizing LBPs (sLBPs), sLBP₁-sLBP₉. Their antioxidant activities in vitro were compared by free radical-scavenging test. sLBP₆, sLBP₈ and sLBP₉ presented stronger activity. In vivo test, 14-day-old chickens were injected respectively with sLBP₆, sLBP₈ and sLBP₉ taking LBP as control, and serum GSH-Px and SOD activities and MDA content were determined. The results showed that three sLBPs could significantly enhance GSH-Px and SOD activities and decrease MDA content. The actions of sLBPs were significantly stronger than that of unmodified LBP. These results indicated that selenylation modification could significantly enhance the antioxidant activities of LBP, sLBP₆ possessed the best efficacy and could be exploited into an antioxidant. The optimal modification conditions were 400 mg of sodium selenite for 500 mg of LBP, reaction temperature of 70 掳C and reaction time of 6 h.