Effect of acetone extract from stem bark of Acacia species (A. dealbata, A. ferruginea and A. leucophloea) on antioxidant enzymes status in hydrogen peroxide-induced HepG2 cells
详细信息 查看全文
- 作者：Kandhasamy Sowndhararajan
- 关键词：Antioxidant enzyme ; Acacia ; Catalase ; HepG2 cells ; Superoxide dismutase
- 刊名：Saudi Journal of Biological Sciences
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：22
- 期：6
- 页码：685-691
- 全文大小：1649 K
文摘
Acacia species are multipurpose trees, widely used in the traditional systems of medicine to treat various ailments. The major objective of the present study was to determine the gene expression of enzymatic antioxidants by acetone extract from the stem bark of three Acacia species (Acacia dealbata, Acacia ferruginea and Acacia leucophloea) in hydrogen peroxide (Hub>2 ub>Oub>2 ub>)-induced human hepatoma (HepG2) cells. The expression of antioxidant enzymes such as superoxide dismutase containing copper–zinc (CuZnSOD)/manganese (MnSOD), catalase (CAT) and glutathione peroxidase (GPx) in HepG2 cells was evaluated by real-time PCR. The results of antioxidant enzyme expression in real-time PCR study revealed that the Hub>2 ub>Oub>2 ub> (200 渭M) challenged HepG2 cells reduced the expression of enzymes such as SOD, GPx and CAT. However, the cells pre-treated with acetone extracts of all the three Acacia species significantly (P > 0.05) up-regulated the expression of antioxidant enzymes in a concentration dependent manner (25, 50 and 75 渭g/mL). In conclusion, the findings of our study demonstrated that the acetone extract of Acacia species effectively inhibited Hub>2 ub>Oub>2 ub> mediated oxidative stress and may be useful as a therapeutic agent in preventing oxidative stress mediated diseases.