Enolase of Mycobacterium tuberculosis is a surface exposed plasminogen binding protein

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• 作者：Amit Rahi¹ ; ^{amitrahi84@gmail.com} ; Sumit Kumar Matta¹ ; ^{sumitmatta@yahoo.co.in} ; Alisha Dhiman¹ ; ^{alisha.dhiman1986@gmail.com} ; Jaishree Garhyan² ; ^{jgarhyan@gmail.com} ; Monisha Gopalani² ; ^{monishasbt@gmail.com} ; Subhash Chandra³ ; ^{scjnu@yahoo.co.in} ; Rakesh Bhatnagar ; ^{rakeshbhatnagar@jnu.ac.in}
• 关键词：Mtb ; Mycobacterium tuberculosis ; TB ; Tuberculosis ; rEno ; recombinant enolase ; MMP ; Matrix Metalloproteinases ; ECM ; Extracellular Matrix
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1861
• 期：1
• 页码：3355-3364
• 全文大小：1355 K
• 卷排序：1861

文摘

Mtb enolase is surface exposed and binds human plasminogen with high affinity. Anti-Mtb enolase antibodies can be detected in sera of TB patients. C-terminal lysine substitution in rEno decreased its binding to human plasminogen. Enolase bound plasminogen undergoes conversion into plasmin. Plasminogen binding to Mtb surface was reduced in presence of anti-rEno antibodies. Immunization with rEno led to a significant decrease in lung Mtb CFU counts.