Apoferritin nanocage as streptomycin drug reservoir: Technological optimization of a new drug delivery system

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• 作者：B. Ruozi^a ; P. Veratti^a ; M.A. Vandelli^a ; A. Tombesi^b ; M. Tonelli^b ; F. Forni^a ; F. Pederzoli^a ; D. Belletti^a ; G. Tosi^a ; ^{gtosi@unimore.it}
• 关键词：Apoferritin ; Encapsulation efficiency ; Nanocage ; Purification process
• 刊名：International Journal of Pharmaceutics
• 出版年：2017
• 出版时间：25 February 2017
• 年：2017
• 卷：518
• 期：1-2
• 页码：281-288
• 全文大小：1505 K
• 卷排序：518

文摘

The aim of this study is to formulate and characterize streptomycin-loaded apoferritin nanoparticles (ApoStrep NPs) for their potential therapeutic use in bacterial resistant infections (i.e. tuberculosis). ApoStrep NPs were prepared by disassembly/reassembly process via pH method and changing apoferritin/drug molar ratio, purified by dialyses process also associated with gel filtration chromatography and characterized in their chemico-physical and technological parameters as yield, size distribution, polidispersivity, morphology, internal structure, zeta potential and loading efficacy. The results showed that spherical reproducible NPs could be obtained by using apoferritin/drug molar ratio lower than 1:25 and purification based on the combination of dialysis and gel filtration chromatography. Photon correlation spectroscopy, Uv–visible detection and electron microscopy showed the maintenance of the native apoferritin chemico-physical properties and structure. When formulated with apoferritin/drug 1:10 and 1:25 molar ratio, ApoStrep NPs showed remarkable encapsulation efficacy (35% and 28%, respectively) along with kinetic profile of drug delivery, approximately 15% at 37 °C in 72 h, as evidenced by “in vitro” release experiments.