Dual-mode optical microscope based on single-pixel imaging

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• 作者：A.D. Rodrí ; guez^a ; ; P. Clemente^b ; E. Tajahuerce^a ; J. Lancis^a
• 关键词：Microscopy ; Reflection ; Transmission ; Resolution ; Computational imaging
• 刊名：Optics and Lasers in Engineering
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：82
• 期：Complete
• 页码：87-94
• 全文大小：1476 K

文摘

We demonstrate an inverted microscope that can image specimens in both reflection and transmission modes simultaneously with a single light source. The microscope utilizes a digital micromirror device (DMD) for patterned illumination altogether with two single-pixel photosensors for efficient light detection. The system, a scan-less device with no moving parts, works by sequential projection of a set of binary intensity patterns onto the sample that are codified onto a modified commercial DMD. Data to be displayed are geometrically transformed before written into a memory cell to cancel optical artifacts coming from the diamond-like shaped structure of the micromirror array. The 24-bit color depth of the display is fully exploited to increase the frame rate by a factor of 24, which makes the technique practicable for real samples. Our commercial DMD-based LED-illumination is cost effective and can be easily coupled as an add-on module for already existing inverted microscopes. The reflection and transmission information provided by our dual microscope complement each other and can be useful for imaging non-uniform samples and to prevent self-shadowing effects.