Development of a RapidFire mass spectrometry assay and a fluorescence assay for the discovery of kynurenine aminotransferase II inhibitors to treat central nervous system disorders

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• 作者：Hao Lu^a ; ^{hao.lu@bms.com" class="auth_mail" title="E-mail the corresponding author} ; Lisa Kopcho^a ; Kaushik Ghosh^b ; Mark Witmer^c ; Michael Parker^d ; Sumit Gupta^b ; Marilyn Paul^b ; Prasad Krishnamurthy^b ; Basanth Laksmaiah^b ; Dianlin Xie^c ; Jeffrey Tredup^c ; Litao Zhang^a ; Lynn M. Abell^a ; ^{lynn.abell@bms.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：KATII ; RapidFire ; Mass spectrometry ; Fluorescence ; High throughput
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：15 May 2016
• 年：2016
• 卷：501
• 期：Complete
• 页码：56-65
• 全文大小：2322 K

文摘

Kynurenine aminotransferases convert kynurenine to kynurenic acid and play an important role in the tryptophan degradation pathway. Kynurenic acid levels in brain have been hypothesized to be linked to a number of central nervous system (CNS) disorders. Kynurenine aminotransferase II (KATII) has proven to be a key modulator of kynurenic acid levels in brain and, thus, is an attractive target to treat CNS diseases. A sensitive, high-throughput, label-free RapidFire mass spectrometry assay has been developed for human KATII. Unlike other assays, this method is directly applicable to KATII enzymes from different animal species, which allows us to select proper animal model(s) to evaluate human KATII inhibitors. We also established a coupled fluorescence assay for human KATII. The short assay time and kinetic capability of the fluorescence assay provide a useful tool for orthogonal inhibitor validation and mechanistic studies.