A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate

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• 作者：Odile Francesca Restaino^a ; ^{odilefrancesca.restaino@unina2.it} ; Rosario Finamore^a ; ^{rosario.finamore@unina2.it} ; Paola Diana^a ; ^{paola.diana@unina2.it} ; Mariacarmela Marseglia^a ; ^{marimars84@hotmail.it} ; Mario Vitiello^a ; ^{mariovitiello.ita@gmail.com} ; Angela Casillo^b ; ^{angela.casillo@unina.it} ; Emiliano Bedini^b ; ^{emiliano.bedini@unina.it} ; Michelangelo Parrilli^c ; ^{michelangelo.parrilli@unina.it} ; Maria Michela Corsaro^b ; ^{mariamichela.corsaro@unina.it} ; Marco Trifuoggi^b ; ^{marco.trifuoggi@unina.it} ; Mario De Rosa^a ; ^{mario.derosa@unina2.it} ; Chiara Schiraldi^a ; ^{chiara.schiraldi@unina2.it}
• 关键词：Chondroitin sulfate ; Keratan sulfate ; GC-MS ; HPAE-PAD ; Pharmacopeia methods ; SEC-TDA
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：15 March 2017
• 年：2017
• 卷：958
• 期：Complete
• 页码：59-70
• 全文大小：2730 K
• 卷排序：958

文摘

A multi-analytical approach was set up, for the first time, for the determination of the residual keratan sulfate contamination in chondoitin sulfate samples. A new HPAED method was developed, compared to a GC-MS one and used to screen standard and commercial samples. Keratan sulfate contamination was found in the range from 0.1 to 19%, in almost 50% of the samples tested it was over the 5% limit. First time detected keratan sulfate contamination in both bovine and marine origin standards. SEC-TDA analyses, SAX chromatography and NMR studies allowed to detect in chondrotin sulfate samples the presence of low sulfated chains and proteoglycan bounded ones.