Synthesis of haptens and development of an indirect enzyme-linked immunosorbent assay for tris(2,3-dibromopropyl) isocyanurate

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• 作者：Lei Shi ; Hongyan Feng ; Pingjing Zhang ; Liping Zhou ; Dexun Xie ; Delie An ; Qingyun Cai
• 关键词：Tris(2 ; 3-dibromopropyl) isocyanurate ; ELISA ; Polyclonal antibody ; Haptens
• 刊名：Analytical Biochemistry
• 出版年：15 February, 2014
• 年：2014
• 卷：447
• 期：Complete
• 页码：15-22
• 全文大小：1480 K

文摘

A competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed for detection of tris(2,3-dibromopropyl) isocyanurate (TBC). Polyclonal antibodies against TBC were raised from synthesized haptens and then screened against various coating antigens. After optimization of the immunoassay conditions, the linear range and IC₅₀ value of the assay were 0.30-100 and 5.17 渭g/L, respectively, with little cross-reactivity (猢?%). Recovery of various samples (water, serum, soil) was tested and the values ranged from 68% to 110%. This ciELISA was also applied to determine TBC in the riverside soil of the Liuyang River, and the results were compared with the data obtained by UHPLC-MS/MS. The experimental assay results confirmed that this proposed immunoassay is a specific, sensitive, and reliable method for determination and monitoring of TBC.