Exploring the potential of natural bacterial consortium to degrade mixture of dyes and textile effluent

详细信息    查看全文

• 作者：Swati M. Joshi ; Shrirang A. Inamdar ; Amar A. Telke ; Dhawal P. Tamboli ; Sanjay P. Govindwar
• 关键词：TGGE ; T-RFLP ; Natural consortium ; Dye mixture ; Decolorization
• 刊名：International Biodeterioration and Biodegradation
• 出版年：2010
• 出版时间：October 2010
• 年：2010
• 卷：64
• 期：7
• 页码：622-628
• 全文大小：553 K

文摘

Mixture of 8 textile dyes was degraded in nutrient broth by using natural bacterial consortium within 3.5 h at static condition. The same consortium effectively degraded textile industry effluent within 3 h. ADMI (American Dye Manufacturer’s Institute) value removal was 89 % and 67 % for dye mixture and industrial effluent, respectively. The optimum pH and temperature for the decolorization of dye mixture was found to be 5 and 50 °C, respectively. Significant induction of intracellular enzymes laccase, azo reductase, veratryl alcohol oxidase and DCIP (2,6-dichlorophenol-indophenol) reductase suggested their active role in dye decolorization. Presence of 6 bacteria in natural consortium obtained from textile dye disposal site was confirmed by using molecular techniques, namely, T-RFLP (Terminal Restriction Fragment Length Polymorphism) and TGGE (Temperature Gradient Gel Electrophoresis). The products formed after decolorization of dye mixture were analyzed by FTIR spectroscopy, HPLC, HPTLC and GC–MS.