Extraction, characterization and evaluation of Kaempferia galanga L. (Zingiberaceae) rhizome extracts against acute and chronic inflammation in rats

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• 作者：Puralae Channabasavaiah Jagadish^a ; Kotehal Parameshwarappa Latha^b ; Jayesh Mudgal^c ; Gopalan Kutty Nampurath^c ; ^{ng.kutty@manipal.edu}
• 关键词：Diclofenac (PubChem CID ; 5018304) ; Ethyl p-methoxycinnamate (PubChem CID ; 5281783)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：24 December 2016
• 年：2016
• 卷：194
• 期：Complete
• 页码：434-439
• 全文大小：832 K
• 卷排序：194

文摘

The rhizomes of an acaulescent perennial herb, Kaempferia galanga Linn (Family: Zingiberaceae), used as traditional ayurvedic herb to get relief from indigestion, swelling, pain, high blood pressure and dyslipidemia.Aim of the studyTo prepare and characterize various extracts of Kaempferia galanga (K. galanga) for their comparative evaluation for the identification of the most efficacious extract and its possible pharmacological implication in acute and chronic inflammatory paradigm.Materials and methodsDried and powdered rhizome of K. galanga was subjected to alcoholic extraction as well as successive extractions with various solvents. After phytochemical characterization, all the extracts were standardized for the presence of ethyl-p-methoxycinnamate. The extracts, and the isolated compound, were tested against carrageenan-induced acute inflammation in rats. The most promising extract was tested against adjuvant-induced chronic inflammation in rats. Further, local myeloperoxidase (MPO) levels were investigated to establish the possible mechanism of action.ResultsAmong the extracts, petroleum ether extract (SKG-1) and crude alcoholic extract (KG) had the maximum quantity of ethyl-p-methoxycinnamate. SKG-1 (300 mg/kg) was found effective against acute inflammation in rats. Further, SKG-1 (100 mg/kg) reversed the inflammation and elevated MPO levels found in the chronic model.ConclusionsThe results suggest that among all the extracts of K. galanga, SKG-1 effectively suppresses the progression of acute and chronic inflammation in rats by inhibition of neutrophil infiltration.