Construction and characterization of a pure protein hydrogel for drug delivery application

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• 作者：Xu Xu^a ; ^b ; ZhaoKang Xu^b ; XiaoFeng Yang^a ; YanHao He^a ; Rong Lin^a ; ^{linrong@mail.xjtu.edu.cn}
• 关键词：Hydrogel ; Recombinant proteins ; Drug delivery ; In vitro release
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：294-298
• 全文大小：1407 K
• 卷排序：95

文摘

Injectable hydrogels have a variety of applications, including regenerative medicine, tissue engineering and controlled drug delivery. In this paper, we reported on a pure protein hydrogel based on tetrameric recombinant proteins for the potential drug delivery application. This protein hydrogel was formed instantly by simply mixing two recombinant proteins (ULD-TIP1 and ULD-GGGWRESAI) through the specific protein-peptide interaction. The protein hydrogel was characterized by rheology and scanning electron microscopy (SEM). In vitro cytotoxicity test indicated that the developed protein hydrogel had no apparent cytotoxicity against L-929 cells and HCEC cells after 48 h incubation. The formed protein hydrogels was gradually degraded after incubation in phosphate buffered solution (PBS, pH = 7.4) for a period of 144 h study, as indicated by in vitro degradation test. Encapsulation of model drug (sodium diclofenac; DIC) were achieved by simple mixing of drugs with hydrogelator and the entrapped drugs was almost completely released from hydrogels within 24 h via a diffusion manner. As a conclusion, the simple and mild preparation procedure and good biocompatibility of protein hydrogel would render its good promising candidate for drug delivery applications.