Vitrification versus slow freezing of oocytes: effects on morphologic appearance, meiotic spindle configuration, and DNA damage

详细信息    查看全文

• 作者：Mó ; nica Mart&#xed ; nez-Burgos ; Leyre Herrero ; Diego Meg&#xed ; as ; Rubé ; n Salvanes ; Mar&#xed ; a C. Montoya ; Ana C. Cobo ; Juan A. Garcia-Velasco
• 关键词：Vitrification ; slow freezing ; cellular volume ; meiotic spindle ; DNA fragmentation
• 刊名：Fertility and Sterility
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：95
• 期：1
• 页码：374-377
• 全文大小：83 K

文摘

To assess possible effects on subcellular organization after cryopreservation, we compared vitrified and slowly frozen oocytes in terms of their post-warm/thaw morphology, meiotic spindle configuration, and DNA integrity. DNA integrity of cryopreserved oocytes was not altered after the procedures, but vitrification was more effective than slow cooling, as shown by higher survival rate and spindle assessment despite a higher misalignament between meiotic spindle and polar body.