Hydrophilic interaction chromatography coupled to tandem mass spectrometry in the presence of hydrophilic ion-pairing reagents for the separation of nucleosides and nucleotide mono-, di- and triphosphates
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- 作者：Marí ; a Mateos-Vivas ; Encarnació ; n Rodrí ; guez-Gonzalo ; erg@usal.es" class="auth_mail" title="E-mail the corresponding author ; Diego Garcí ; a-Gó ; mez ; Rita Carabias-Martí ; nez
- 关键词：Hydrophilic interaction chromatography (HILIC) ; Hydrophilic ion-pairing ; Nucleosides ; Nucleotide mono- ; di- and triphosphates
- 刊名：Journal of Chromatography A
- 出版年：2015
- 出版时间：2 October 2015
- 年：2015
- 卷：1414
- 期：Complete
- 页码：129-137
- 全文大小：1161 K
文摘
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Nucleotides are not retained in unbuffered HILIC because of a repulsion mechanism.
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Hydrophilic cationic ion-pairing reagents enhance this retention.
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Retention increases with the polarity of the cationic ion-pairing reagent.
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The retention for these ion-pairs is based on a partition mechanism.
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20 Nucleosides and nucleotides can be separated by HILIC in less than 9 min.