Diepoxybutane interstrand cross-links induce DNA bending

详细信息    查看全文

• 作者：Julie T. Millard ; ^{jtmillar@colby.edu} ; Erin E. McGowan ; Sharonda Q. Bradley
• 关键词：DNA cross-linking ; Diepoxybutane ; DNA bending ; DNA damage ; Alkylating agent
• 刊名：Biochimie
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：94
• 期：2
• 页码：574-577
• 全文大小：456 K

文摘

The bifunctional alkylating agent 1,2,3,4-diepoxybutane (DEB) is thought to be a major contributor to the carcinogenicity of 1,3-butadiene, from which it is derived in?vivo. DEB forms DNA interstrand cross-links primarily between distal deoxyguanosine residues at the duplex sequence 5?GNC. In order for the short butanediol tether to span this distance, distortion of the DNA target has been postulated. We determined that the electrophoretic mobility of ligated DNA oligomers containing DEB cross-links was retarded in comparison with control, uncross-linked DNA. Our data are consistent with DNA bending of ?4¡ã?per?lesion towards the major groove.