Characterisation of two self-sufficient CYP102 family monooxygenases from Ktedonobacter racemifer DSM44963 which have new fatty acid alcohol product profiles
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- 作者：Samuel D. Munday ; Natasha K. Maddigan ; Rosemary J. Young ; Stephen G. Bell ; stephen.bell@adelaide.edu.au" class="auth_mail" title="E-mail the corresponding author
- 关键词：BSTFA ; N ; O-bis(trimethylsilyl)trifluoroacetamide ; CYP ; cytochrome P450 enzyme ; DEAE ; diethylaminoethanol ; DMSO ; dimethyl sulfoxide ; EMM ; Escherichia coli minimal media ; EtOH ; ethanol ; GC ; gas chromatography ; HPLC ; high performance liquid chromatography ; IPTG ; isopropyl β-D-1-thiogalactopyranoside ; MS ; mass spectrometry ; NADH ; reduced Nicotinamide adenine dinucleotide ; NADPH ; reduced Nicotinamide adenine dinucleotide phosphate ; PCR ; polymerase chain reaction ; TMSCl ; trimethylsilyl chloride ; Tris
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：1860
- 期：6
- 页码：1149-1162
- 全文大小：1860 K
文摘
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Identification of two self-sufficient cytochrome P450 genes in K. racemifer DSM44963
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Analysis of the relationship of Krac0936 and Krac9955 with other P450Bm3 like enzymes
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Purification and preliminary characterisation of both enzymes
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The observation of modified selectivity of fatty acid oxidation by Krac0936 compared to P450Bm3
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Krac9955 has an unusual substrate range and product selectivity.