Evolutionary, Mechanistic, and Predictive Analyses of the Hydroxymethyldihydropterin Pyrophosphokinase Family of Proteins

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• 作者：Gerloff ; Dietlind L. ; Cannarozzi ; Gina M. ; Joachimiak ; Marcin ; Cohen ; Fred E. ; Schreiber ; David ; et. al.
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：1999
• 出版时间：January 8, 1999
• 年：1999
• 卷：254
• 期：1
• 页码：70-76
• 全文大小：214 K

文摘

A prediction has been preparedab initiofor the secondary structure of the hydroxymethyldihydropterin pyrophosphokinase (HPPK) family of proteins starting from a set of aligned homologous protein sequences. Attempts to identify a fold by threading failed, judging by the inability to find a threading “hit” that had a secondary structure that was plausibly congruent to the predicted secondary structure for the HPPK family. Therefore, a set of tertiary structure models was assembledab initio,where alternative models were built and used to select between alternative secondary structure models. This prediction report illustrates the importance of non-computational approaches to structure prediction at its present frontier, which is to obtain medium resolution models of tertiary structure.