Umbilical cord-derived mesenchymal stromal cell-conditioned medium exerts in vitro antiaging effects in human fibroblasts

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• 作者：Meirong Li¹ ; ² ; ^* ; Yali Zhao¹ ; ² ; ^* ; Haojie Hao¹ ; Liang Dong¹ ; Jiejie Liu¹ ; Weidong Han¹ ; ^{hanwdrsw69@yahoo.com} ; Xiaobing Fu¹ ; ^{fuxiaobing@vip.sina.com}
• 关键词：chronic wounds ; high glucose ; myofibroblast ; mesenchymal stromal cell-conditioned medium ; senescence ; oxidative stress
• 刊名：Cytotherapy
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：19
• 期：3
• 页码：371-383
• 全文大小：3257 K
• 卷排序：19

文摘

Chronic wounds are a common complication of diabetes. Fibroblast-myofibroblast differentiation is important for wound repair, which is commonly impaired in non-healing wounds, and the underlying mechanisms need to be further elucidated.MethodsWe used high glucose (HG) to simulated the diabetes microenvironment and explored its effects on the biological features of fibroblasts in vitro.ResultsThe results showed that prolonged HG induced senescence in fibroblasts through activation of p21 and p16 in a reactive oxygen species (ROS)-dependent manner, further delayed the viability and migration in fibroblasts and also depressed fibroblast differentiation through the TGF-β/Smad signaling pathway. However, mesenchymal stromal cell–conditioned medium (MSC-CM) counteracts the effects of HG. Treatment of fibroblasts with MSC-CM decreased HG-induced ROS overproduction, ameliorated HG-induced senescence in fibroblasts and reversed the defects in myofibroblast formation. Our results may provide clues for the pathogenesis of chronic wounds and a theoretical basis to develop MSC-CM as an alternative therapeutic method to treatment of chronic wounds.